Abstract
Selective-plane illumination microscopy (SPIM) are particularly advantageous in long-term live volumetric imaging, although its common geometry is incompatible with common biological sample holders, including multi-well plates. To solve this problem, we designed an epi-illumination SPIM (eSPIM) system with high spatial resolution and light collection efficiency with single-molecule sensitivity. It has an identical sample interface as an inverted fluorescence microscope with a single primary objective and no additional reflection elements. We have demonstrated multicolor, fast, volumetric imaging of live cells as well as single-molecule super-resolution microscopy on eSPIM. Moreover, it has enabled long term imaging of cells in parallel in multiwell plates and simultaneous recording of cellular responses to a panel of perturbations.
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