Epigenetic Topography of Lymphocyte Nuclei and Endothelin-1 at Atherosclerosis

Abstract

to analyze to cytokines, sCAM secretion within chromatin topography of lymphocytes at atherosclerosis. Two kinds of response were researched: coagulation (incubation of blood clot within 6 hrs at 37 C) and standardized viscosimetric flow rotational viscosimetr (shear rate 100 -1/s, 60 s at 37 C incubation within 6 hrs at 37 C). ET-1 and sP-, sE-selectin's, sICAM-1 (R&D, UK) were determined by ELISA kits (photometer "Biotech-1000", Beckman, U.S.A.). The chromatin of lymphocyte nuclei was studied using Computer TV Morphodensitometry System "DiaMorph"(Russia) in the smears dyed especially for DNA. After rheological test the decrease of chromatin activity happened. The significant decrease of selectin level's closely correlated to increased of ET-1 level. We observed the deep reorganization of nuclei after the coagulation test (proapoptotic?). ET-1 did not protect lymphocytes from chromatin changes even enchansed the chromatin inactivation. We established that the lymphocytes are the active participants in pro- and antiinflammatory cytokines. We hereby hypothesize that the unclear cell communication mechanisms of observed phenomena were triggered.