Abstract
Aim: To assess isorhamnetin efficacy for diabetic kidney disease in aType 2 diabetes mellitus rat model, through investigating its effect at theepigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50mg/kg/d) for 4 or 8weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (FYCO1, ULK, TECPR1 andWIPI2)and miR-15b, miR-34a and miR-633 were assessed byqRT-PCR, and LC3A/B by immunoblotting. Results: Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNAregulation of autophagy genes. Conclusion: We proposea molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.
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