Abstract
The dataset consists of two comma-delimited plain text files for global methylation and MS-AFLP data, respectively. In each file, each row refers to an individual module. For MS-AFLP columns refers to a specific (although anonymous) marker, identified by primer combination and size (base pairs). Three L. latifolia shrubs were sampled at a large population growing near Arroyo Aguaderillos in the Sierra de Cazorla (Jaen province, southeastern Spain; geographical coordinates 37.96157 N, 2.88389 W). Fresh leaf samples were collected from as many individual modules as possible in each plant. DNA cytosine methylation was determined for each sample by reversed phase HPLC with spectrofluorimetric detection. Global cytosine methylation was estimated as 100 x 5mdC/(5mdC + dC), where 5mdC and dC are the integrated areas under the peaks for 5-methyl-2'-deoxycytidine and 2'-deoxycytidine, respectively. The MS-AFLP analyses were performed using standard protocols involving the use of fluorescent dye-labeled selective primers. Each sample was fingerprinted using eight primer combinations, each with two (HpaII) or three (MseI) selective nucleotides. Fragment separation and detection was made using an ABI PRISM 3130xl DNA sequencer, only fragments ≥ 150 base pairs in size were considered and the presence (1) or absence (0) of fragments in each sample was scored manually by visualizing electropherograms with GeneMapper 3.7 software
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.