Abstract
An understanding of cellular processes that determine the response to ionizing radiation (IR) treatment is essential to improve radiotherapy. We investigated the DNA methylation changes in X‐ray‐irradiated TK6 and WTK1 cells that differ in sensitivity to IR. The global DNA methylation alterations as measured by an ELISA showed hypomethylation. Using AP‐PCR we observed time dependent dynamic changes in the regional genomic DNA methylation patterns. The AP‐PCR profiles were different between these cells indicating the involvement of differential genomic DNA responses to radiation treatment. The analysis of the components of the DNA methylation machinery showed the modulation of maintenance and de novo methyltransferases in irradiated cells. DNMT1 mRNA levels were increased in TK6 cells but were repressed in WTK1 cells. DNMT3A and DNMT3B were induced in both cells. TET1, involved in the conversion of 5‐methylcytosine to 5‐hydroxymethylcytosine was induced in both cells. This study demonstrates that irradiated cells acquire epigenetic changes in the DNA methylation patterns and the associated cellular machinery are involved in the response to radiation exposure. This study also shows that DNA methylation patterns are changed at different genomic regions and are dependent upon time after irradiation and the genetic background of the cell.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
