Abstract
Introduction: The rise in antibiotic resistant cases has caused a global concern. Researchers around the world are trying to find a novel alternative to combat this issue. Green tea with its many health benefits, including antibacterial and antiviral activity, has shown to be one of the most promising candidates to be used as an agent to solve this problem. Objective: This study focuses on evaluating the synergistic effects of antibiotics and two green tea polyphenols: epigallocatechin gallate (EGCG), and its modified lipophilic form epigallocatechin gallate stearate (EGCG-S). Methods: In this study, twelve antibiotics and eight bacteria: Gram-positive Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis) and Bacillus megaterium (B. megaterium); Gram-negative Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Serratia marcescens (S. marcescens), and Enterobacter aerogenes (E. aerogenes); and acid-fast Mycobacterium smegmatis (M. smegmatis) were used. Antibacterial synergism profiling of EGCG, EGCG-S and antibiotics has been established using a disk diffusion assay. Results: The results revealed that both 1% of EGCG and 1% EGCG-S enhanced the antimicrobial activities on antibiotics in various bacteria. Antimicrobial susceptibility study indicated that EGCG-S was able to enhance some antibiotics from the resistant category to intermediate or susceptible and/or from intermediate category to susceptible. Both EGCG and EGCG-S worked comparably on Gram-positive bacteria; in S. aureus, both compounds enhanced 5 antibiotics (AM10, CF30, C30, S10 and TE30) activities while EGCG-S had higher efficiency. B. megaterium were susceptible to most of the antibiotic treatment, thus the impact of EGCG and EGCG-S was insignificant. EGCG-S worked better than EGCG on Gram-negative bacteria; converted 9 antibiotics susceptibility in E. coli and P. aeruginosa, and 8 antibiotics in E. aerogenes. EGCG and EGCG-S also showed synergism on acid-fast bacteria M. smegmatis with EGCG-S has much higher efficiency than EGCG. Conclusion: The results suggested that EGCG-S might be a promising antibacterial synergistic agent with antibiotics to combat antibiotic-resistant bacteria.
Highlights
The rise in antibiotic resistant cases has caused a global concern
From the zone of inhibition (ZOI) study, B. megaterium was generally susceptible to antibiotic treatments; 10 of 12 antibiotics work on this organism
These results indicate that epigallocatechin gallate (EGCG)-S may be a synergistic agent in enhancing the antimicrobial activity of antibiotics
Summary
The rise in antibiotic resistant cases has caused a global concern. Researchers around the world are trying to find a novel alternative to combat this issue. Antimicrobial susceptibility study indicated that EGCG-S was able to enhance some antibiotics from the resistant category to intermediate or susceptible and/or from intermediate category to susceptible Both EGCG and EGCG-S worked comparably on Gram-positive bacteria; in S. aureus, both compounds enhanced 5 antibiotics (AM10, CF30, C30, S10 and TE30) activities while EGCG-S had higher efficiency. The extensive global use of antibiotics, lack of new and effective antibiotics and increased spread of multi-drug resistant bacteria have led to the looming threat to global health [2]. This rapid accumulation of antibiotic resistance suggests a reservoir of transferable antibiotic resistance gene determinants in response to antibiotics that were found in hospitals, environmental isolates, and even in permafrost DNA [3]. With multi-drug resistance representing one of the top threats to public health globally, novel natural alternatives are tested to help combat the rise of antibiotic resistant bacteria
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
