Epigallocatechin gallate increase the prostacyclin production of bovine aortic endothelial cells☆

Abstract

We describe the effect of (−) epigallocatechin gallate (EGCg), one of catechins known in tea, on the prostacyclin (PGI) production by bovine aortic endothelial cells. The amounts of 6-keto-PGF 1α and Δ 17-6-keto-PGF 1α, stable metabolites of PGI 2 and PGI 3, released in culture medium were measured using gas chromatography/selected ion monitoring (GC/SIM). The prostacyclin production of endothelial cells was increased by EGCg in a dose- and time-dependent manner. The effect by EGCg was stronger than any other catechins (catechin, epicatechin, epigallocatechin, and epicatechin gallate). When endothelial cells incubated with EGCg and arachidonic acid (AA) or eicosapentaenoic acid (EPA), PGI 2, and PGI 3 production were increased greater than those incubated with AA or EPA alone. Furthermore, gallic acid, that also has a pyrogallol structure, increased PGI 2 production. These observations indicate that catechins increase the prostacyclin production and that the pyrogallol structure is significant to this function.