Epidermolysis bullosa acquisita: the most frequent pemphigoid disease in patients with dermal binding autoantibodies by indirect immunofluorescence microscopy on human salt-split skin in Tehran, Iran.

Abstract

Pemphigoid diseases (PD) are characterized by autoantibodies targeting structural proteins of the basement membrane zone 1 . The diagnosis of some PD may be challenging despite the use of indirect immunofluorescence (IIF) method on salt-split human skin (SSS) and serration pattern analysis by direct immunofluorescence microscopy of perilesional biopsies. Here, we prospectively recruited 21 consecutive patients with PD diagnosed by a compatible clinical blistering phenotype, linear deposits of IgG and/or C3 by direct immunofluorescence (DIF), and binding of IgG serum autoantibodies to the dermal side of SSS by IIF presenting from December 2017 until January 2019 to Razi Hospital, Tehran, Iran (Figure 1).