Epidermal Growth Factor Is Associated with Loss of Mucosae Sealing and Peri-Implant Mucositis: A Pilot Study.

José Jorge Schoichet,Priscila Casado,Marina Prado,Edgard De Mello Fonseca,Rafael Mello-Machado,Carlos Ramirez,Angelo Cardarelli,Juliana Prazeres,Ricardo Villas-Boas,Carlos Fernando De Almeida Barros Mourão,Valquiria Quinelato,Telma Regina Aguiar

doi:10.3390/healthcare9101277

José Jorge Schoichet, Priscila Casado + Show 10 more

Open Access

https://doi.org/10.3390/healthcare9101277

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Abstract

This study aimed to evaluate the correlation between epidermal growth factor (EGF) and receptor (EGFR) levels in different clinical stages of dental implant rehabilitation and trace mucositis development’s biological profile. Thirty-six participants from the Specialization in Implant Dentistry, Universidade Federal Fluminense, Brazil, were included in the study and underwent sample collection: inside the alveolar socket, immediately before implant placement (Group 1, n = 10); at the peri-implant crevicular fluid (PICF) during reopening (Group 2, n = 10); PICF from healthy peri-implant in function (Group 3, n = 8); and PICF from mucositis sites (Group 4, n = 18). Quantitative polymerase chain reaction (PCR) evaluated EGF/EGFR gene expression using the SYBR Green Master Mix detection system. The results showed that EGF expression in the peri-implant crevicular fluid was statistically different. There was a higher EGF expression for group C (peri-implant health) (p = 0.04) than for the other groups. Regarding EGFR, there was no statistical difference among the groups (p = 0.56). It was concluded that low levels of EGF gene expression in the peri-implant crevicular fluid are related to the development of peri-implant mucositis and the absence of mucosae sealing. There was no correlation between EGFR gene expression with health or mucositis.

Highlights

Implant therapy has been widely applied in oral rehabilitation for many years, with predictable long-term results
Based on the biological function of EGF and the Healthcare 2021, 9, 1277 increased incidence of peri-implant mucositis, this study aimed to evaluate the correlation between the levels of epidermal growth factor (EGF) and its receptor (EGFR) at different stages
Considering EGF with its receptor (EGFR) gene expression levels, the results showed no statistically significant differences among the groups (p = 0.56) (Figure 3)

Summary

Introduction

Implant therapy has been widely applied in oral rehabilitation for many years, with predictable long-term results. The longevity and functionality of dental implants depend on the new bone formation around the implant body and the establishment of a soft tissue barrier called mucosal sealing that protects the underlying peri-implant structures and the implant itself. The presence of peri-implant mucosal inflammation (mucositis) can lead to loss of mucosal sealing and possible pathological bone resorption (peri-implantitis), culminating in implant loss [1]. Peri-implant diseases have become increasingly present in daily clinical practice. Many risk factors are associated with mucositis development, such as smoking habit, poor oral hygiene, history of periodontitis, alcohol use, long-term use of non-steroidal anti-inflammatory drugs, bisphosphonates, and uncontrolled diabetes Type 2 [3]

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