EPIDEMIOLOGY STUDY OF VIRAL NERVOUS NECROSIS (VNN) IN MALAYSIAN GROUPERS: SEQUENCE ANALYSIS STRATEGIES AND QUASISPECIES DETERMINANT’S

Abstract

Event Abstract Back to Event EPIDEMIOLOGY STUDY OF VIRAL NERVOUS NECROSIS (VNN) IN MALAYSIAN GROUPERS: SEQUENCE ANALYSIS STRATEGIES AND QUASISPECIES DETERMINANT’S Sandra C. Zainathan1*, Azila Abdullah2, Mohammed Noor Amal Azmai3, Nurshuhadah Ariff1 and Najiah Musa4 1 Universiti Malaysia Terengganu, Institute of Marine Biotechnology, Malaysia 2 National Fish Health Research Centre, Lab, Malaysia 3 Universiti Putra Malaysia, Biology Dept Faculty of Science, Malaysia 4 Universiti Malaysia Terengganu, School of Fisheries and Aquaculture Sciences, Malaysia Viral Nervous Necrosis (VNN) or Viral encephalopathy and retinopathy (VER) is considered to be a serious disease of several marine fish species, characterized by significant losses associated to vacuolating lesions of the central nervous system and the retina. In Malaysia, VNN has been isolated from groupers, seabass, red snappers, cobia and golden pomfret. Betanodaviruses causes 100% mortality in larval stages while lower losses have been reported in juveniles and adult fish. Due to the unsystematic surveillance and reporting system, including risk factors, source of VNN infections; early detection method together with control and prevention strategies in Malaysia are not fully determined yet. VNN is reported to be vertically transmitted and it is still unclear how the virus is transmitted from broodstocks to fry/fingerlings, and the inconsistencies of detection in the broodstocks itself, especially during breeding time (self-observation). Since it is difficult to obtain and culture grouper broodstocks, hence, early control and prevention should be applied to ensure the broodstocks are protected against this disease. Thus, we propose to study the epidemiology of VNN in broodstocks and cultured grouper with a focus on prevalence, risk factors together with its genome characterization. These information may provide basis for the development of detection methods, vaccine or other control and prevention strategies. The samples (complete life stages) will be collected from associated grouper farms in Malaysia. In this study, risk factors for VNN infections such as age, species, life stages and water quality parameters will be determined. The use of invasive and non-invasive sampling methods will be evaluated as well. The genotypes and prevalence of VNN (seasonally) can be determined in this study. The confirmation of VNN genome sequence will provide information useful for understanding the evolution and divergence of the virus as well as towards the potential vaccine development. Acknowledgements Fundamental Research Grant Scheme, Ministry of Higher Education Malaysia References Athanassopoulou, F., Billinis C., Psychas V. and Karipoglou K. (2003) Viral encephalopathy and retinopathy of Dicentrarchus labrax (L.) farmed in fresh water in Greece. 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EPIDEMIOLOGY STUDY OF VIRAL NERVOUS NECROSIS (VNN) IN MALAYSIAN GROUPERS: SEQUENCE ANALYSIS STRATEGIES AND QUASISPECIES DETERMINANT’S. Front. Vet. Sci. Conference Abstract: AquaEpi I - 2016. doi: 10.3389/conf.FVETS.2016.02.00005 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Apr 2016; Published Online: 14 Sep 2016. * Correspondence: Dr. Sandra C Zainathan, Universiti Malaysia Terengganu, Institute of Marine Biotechnology, Kuala Terengganu, Terengganu, 21030, Malaysia, sandra@umt.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sandra C Zainathan Azila Abdullah Mohammed Noor Amal Azmai Nurshuhadah Ariff Najiah Musa Google Sandra C Zainathan Azila Abdullah Mohammed Noor Amal Azmai Nurshuhadah Ariff Najiah Musa Google Scholar Sandra C Zainathan Azila Abdullah Mohammed Noor Amal Azmai Nurshuhadah Ariff Najiah Musa PubMed Sandra C Zainathan Azila Abdullah Mohammed Noor Amal Azmai Nurshuhadah Ariff Najiah Musa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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