Abstract
GNB airway colonization occurs in one-third of newborns who are mechanically ventilated for longer than 2 weeks. This colonization is associated with significant morbidity and mortality. To ascertain their origin and potential modes of transmission, 52 GNB isolates from 14 ventilated newborns were analyzed by pulsed-field gel electrophoresis (PFGE). These isolates were from 41 serial tracheal cultures obtained (July-October 1997) at the NICU where pts. were located in four contiguous rooms. Routine bacteriology showed that five neonates were colonized with two different GNB and one was colonized with three. PFGE analysis revealed that one pt. acquired two strains and one subtype of Klebsiella (A1,E, E1), and another harbored multiple subtypes of Klebsiella(A,A1,A2,A3). For the remaining pts., colonizations were due to unrelated strains. Table
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