Abstract
BackgroundOur aim was to determine and characterize S. aureus (SA) isolated from infections in newborns for antibiotic resistance, virulence factors, genotypes, epidemiology and antibiotic consumption.MethodsProspective surveillance of infections was conducted. Data about antibiotic treatment were analyzed. Antimicrobial susceptibility was assessed. PCR amplification was used to detect resistance and virulence genes. Typing methods such as PFGE, spa-typing and SCCmec were used.ResultsSA was found to be associated with 6.5% of infections. Methicillin-Resistant Staphylococcus aureus accounted for 32.8% of SA-infections. An incidence of MRSA-infections was 1.1/1000 newborns. MRSA-infections were diagnosed significantly earlier than MSSA-infections in these newborns (14th day vs. 23rd day (p = 0.0194)). MRSA-infections increased the risk of newborn’s death. Antibiotic consumption in both group was similar, but a high level of glycopeptides-usage for MSSA infections was observed.In the MRSA group, more strains were resistant to erythromycin, clindamycin, gentamicin and amikacin than in the MSSA group. Hla gene was present in 93.9% of strains, and seg and sei in 65.3% of strains, respectively. One dominant clone was found among the 14 MRSA isolates. Fifteen strains belonging to SCCmec type IV were spa-t015 and one strain belonging to SCCmec type V was spa-t011.ConclusionsResults obtained in the study point at specific epidemiological situation in Polish NICU (more detailed studies are recommended).High usage of glycopeptides in the MSSA infections treatment indicates the necessity of antimicrobial stewardship improvement and introducing molecular screening for early identification of infections.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-015-0890-3) contains supplementary material, which is available to authorized users.
Highlights
Our aim was to determine and characterize S. aureus (SA) isolated from infections in newborns for antibiotic resistance, virulence factors, genotypes, epidemiology and antibiotic consumption
Systemic infections caused by Staphylococcus aureus (SA) are still common among newborns in neonatal intensive care units (NICUs) [1]
The primary aim of this study was to determine and characterize S. aureus isolates from newborns according to resistance, virulence factors, genotypes and epidemiology
Summary
Our aim was to determine and characterize S. aureus (SA) isolated from infections in newborns for antibiotic resistance, virulence factors, genotypes, epidemiology and antibiotic consumption. Systemic infections caused by Staphylococcus aureus (SA) are still common among newborns in neonatal intensive care units (NICUs) [1]. The primary aim of this study was to determine and characterize S. aureus isolates from newborns according to resistance, virulence factors, genotypes and epidemiology. We assessed the level of antibiotic usage and possible risk factors for SA infection (separately for Methicillin-sensitive SA (MSSA) and Methicillin-resistant SA (MRSA)).
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