Abstract
Porcine cysticercosis is widely distributed in developing countries. Many tools were developed for effective control of the tapeworm in endemic countries. Tongue examination, meat inspection and Ag-ELISA were widely used in epidemiological survey. Both tongue examination and meat inspection are highly specific but less sensitive. To improve performance of AgELISA, unambiguous test based on nanobodies was performed. Immunodiagnostic tests based on PCR, flows through assay (FTA), Surface Enhanced Laser Desorption–Ionization Time of Flight (SELDI-TOF) were also developed. Less data were reported using spatial statistical analysis hence multiples approaches were available for effective epidemiological survey of porcine cysticercosis.
Highlights
Taenia solium is a tapeworm responsible of serious public health problem in developing countries [1,2]
Different techniques have been described to detect antibodies and antigens of T. solium infections in man and pigs: enzyme linked immunosorbent assay (ELISA), dipstick-ELISA, latex agglutination, immunoblot techniques, antigen detection based on sandwich enzyme-linked immunosorbent assay (Ag-ELISA) [39]
Antigens detection based on Ag-ELISA was effectively used to detect porcine and human cysticercosis
Summary
Taenia solium is a tapeworm responsible of serious public health problem in developing countries [1,2]. To improve the performance of Ag-ELISA in the detection of porcine cysticercosis, nanobodies derived from camels was used to develop unambiguous specific tests [19]. The use of PCR in serum for the diagnosis of porcine cysticercosis has a high specificity and the assay did not showed cross-reactivity with toxoplasmosis and trichinellosis This technique is specific when used to test the other pig taeniids, mainly Taenia hydatigena, Taenia asiatica [47]. PCR was used for the diagnostic of porcine cysticercosis after inspection of the carcass [48] To perform this PCR protocol, genomic DNA was extracted from T. solium cysticerci dissected from naturally infected cysticercosis positive pigs.
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