Epidemiological studies of clinical and subclinical ovine mastitis in Awassi sheep in northern Jordan

Abstract

Forty-six Awassi sheep flocks selected by stratified random sampling were subjected to a cross-sectional study to determine the prevalence of intramammary infections, to assess the influence of flock size and parity on the prevalence of somatic cell count (SCC) and to identify major udder pathogens. Of the 3472 udder halves examined, 29.8% had over 10 6 SCC/ml and 0.03% had dry teats due to chronic mastitis. Flocks with 30–49 milking ewes (small flock size) were much younger ( P < 0.001) than flocks with 50–99 ewes (medium) and flocks with ≥ 100 ewes (large). Pairwise analysis of the InSCC of both halves of the udders revealed significant mean differences for small and large flock size ( P < 0.05), and for medium and large flock size ( P < 0.001). Mean InSCC was lower ( P < 0.05) in samples obtained from the left half compared with samples of the right half of the udder. Multiparous ewes had higher ( P < 0.001) mean InSCC than primiparous ewes. Also, ewes with twin lambs had higher ( P < 0.001) mean InSCC in the right half of the udder compared with single-lamb ewes. Samples collected in January (winter) had lower ( P < 0.05) mean InSCC compared with samples collected in June. The most common organisms isolated from subclinical mastitis cases were coagulase-negative Staphylococci (17.8%), E. coli (13.6%), Streptococcus agalactiae (6.8%) and Staphylococcus aureus (6.8%). Of the 46 flocks, 20 were monitored monthly for 9 consecutive months to determine the incidence of clinical mastitis diagnosed by shepherds or/and sheep farmers with major pathogens. The incidence of clinical mastitis (expressed as the number of clinical cases per 100 ewe-months) were 2.1 ± 1.9 (SD), 1.9 ± 1.1, and 1.2 + 2.1 for small, medium and large flocks size strata, respectively. The overall population estimate was 1.7 ± 0.02 cases per 100 ewe-months. The most-common clinical isolates were S. aureus (22% of all clinical isolates) and E. coli (14.2%).