Abstract
This study was aimed to determine the prevalence of rotaviruses in Karbala and Basrah provinces, the genetic reassortment of human and animal rotavirus strains and the novel strains. Rotaviruses were detected by Immunochromatography Test (ICT) then the positive samples were tested by reverse transcription PCR (RT-PCR) using specific primers to VP7 gene, this gene is responsible for VP7 antigen which is responsible for stimulation the immune system to produce neutralizing antibodies. The VP7 gene was implemented in rotavirus vaccine of Iraqi immunization program. The RT PCR results showed that 56.3% (27/48) of samples positive in children under five years of age in Karbala province while Basrah province revealed 58.5% (31/53) positive samples in children whereas samples taken from calves revealed 43.1% (22/51) and 45.5% (25/55) positive samples in Karbala and Basrah provinces respectively. The sequencing of human and animal samples revealed that there was genetic reassortment between human and animal strains while in comparing with international strains there was closely related with Indian and Pakistani human strains. Necessarily for further bioinformatics studies are needed to study the genetic alterations with viral proteins analysis of rotavirus.
Highlights
Reoviridae family contains Rotavirus is a non-enveloped double stranded RNA virus that according phylogenetic analysis resolved into 8 types (RVA to RVH) analysis [1]
Rotaviruses were detected by Immunochromatography Test (ICT) the positive samples were tested by reverse transcription Polymerase Chain Reaction (PCR) (RT-PCR) using specific primers to VP7 gene, this gene is responsible for VP7 antigen which is responsible for stimulation the immune system to produce neutralizing antibodies
The RT PCR results showed that 56.3% (27/48) of samples positive in children under five years of age in Karbala province while Basrah province revealed 58.5% (31/53) positive samples in children whereas samples taken from calves revealed 43.1% (22/51) and 45.5% (25/55) positive samples in Karbala and Basrah provinces respectively
Summary
Reoviridae family contains Rotavirus is a non-enveloped double stranded RNA (dsRNA) virus that according phylogenetic analysis resolved into 8 types (RVA to RVH) analysis [1]. The rotavirus genome consists of 11 dsRNA segments which code for the six structural (VP1-VP4, VP6, VP7), VP4 and VP7 responsible for viral attachment proteins and neutralization antigens [2]. Rotavirus A was detected as one of the highest members and widely studied as one of causes diarrheal infection in infants and young children with life threatening diarrhea internationally. Group A rotavirus (RVA) is responsible for acute dehydration diarrhea in humans and animals. RVA shows wide range diversity and a variety of human strains share genetic and antigenic features with animal origin RVA strains [7]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
