Epidemiological and Molecular Study of Cryptosporidium in Preweaned Calves in Kuwait

Abstract

Simple SummaryCryptosporidiosis is a global, zoonotic disease of concern. Cryptosporidium spp. can infect susceptible hosts via a main fecal–oral route due to cross-contamination of raw food and surface water from reservoir animals in the neighborhood, farms, or slaughter houses, besides some mechanical vectors, such as cockroaches and flies. Cryptosporidium parvum alleles are the most common species infecting children, and its potential reservoir is cattle. Hence, understanding the epidemiology of Cryptosporidium spp. in preweaned calves, along with the diagnosis of the predominant species and subtypes infecting them, can play a role in preventing Cryptosporidium spp. spread in the environment. In this study, Cryptosporidium parvum subtype IIaA15G2R1 was the most dominant Cryptosporidium spp. detected in preweaned calves in Kuwait. This subtype was recorded previously in Kuwaiti children suffering from diarrhea. Maintaining good personal hygiene in humans and reducing, controlling, or eliminating the causal risk factors in preweaned calves is a superb strategy for preventing and narrowing the spread of this disease.Cryptosporidium is a worldwide enteric protozoan parasite that causes gastrointestinal infection in animals, including humans. The most notable species is Cryptosporidium parvum because of its zoonotic importance; it is also the leading cause of cryptosporidiosis in preweaned calves. A cross-sectional study was conducted to determine the prevalence of Cryptosporidium infection, investigate the potential risk factors, and use molecular diagnosis to identify the predominant Cryptosporidium spp. in preweaned calves in Kuwait. Of 175 preweaned calves, Cryptosporidium antigens were detected in 58 (33.1%) using rapid lateral immunochromatography assay (IC). Calves less than one month of age (OR = 4.32, p = 0.0001) and poor hygiene (OR = 2.85, p = 0.0075) were identified as significant risk factors associated with Cryptosporidium infection. Molecular identification revealed that C. parvum (62.8%) was the dominant species infecting preweaned calves in Kuwait. In contrast, C. bovis and C. andersoni were recorded at 5.7% and 2.9%, respectively. All C. parvum gp60 nucleotide sequences were subtype IIaA15G2R1. Calves could be a source of C. parvum infection due to the similarity of the subtypes recorded previously in Kuwaiti children and preweaned calves in this study. Therefore, more research is needed to understand the Cryptosporidium transmission cycle in Kuwait.