EPIDEMIOLOGICAL AND MOLECULAR STUDIES ON RIEMERELLA ANATIPESTIFER INFECTION IN DUCKS

Doha Abd Alrahman Ahmed ,Mostafa Saif Eldin ,Roshita Ibrahim ,Omar Amen

doi:10.21608/avmj.2021.177849

Doha Abd Alrahman Ahmed , Mostafa Saif Eldin + Show 2 more

Open Access

https://doi.org/10.21608/avmj.2021.177849

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Abstract

Infectious serositisis a considerable economic problem in duck industry caused by Riemerella anatipestifer. The current study was conducted to investigate the circulating R. anatipestifer in ducks in Assiut Province and assessing their antimicrobial susceptibility. One-hundred and twenty diseased or freshly dead ducks aging 1-18 weeks were examined. Naturally infected birds showed respiratory, nervous, and locomotor disturbances, and low body weight. R. anatipestifer was detected in 16.6% (20) of birds. Among the bacteriologically positive 20 birds, only 10 could be identified by PCR as R. anatipestifer with a prevalence rate of 8.33%. The sensitivity biogram revealed that all the obtained isolates were sensitive to amoxicillin, doxycycline, and flumequine while resistance to streptomycin, chloramphenicol, ampicillin, erythromycin, spectinomycin, and cephradine was observed. On the basis of MIC, all isolates had 90- 100% sensitivity to doxycycline and amoxicillin, respectively. Experimentally, the isolated R.anatipestifer strains showed pathogenicity to 14-days-old ducklings.

Highlights

Among the global leading problems confronting duck industry is Riemerella anatipestifer infection that implicates in acute and chronic conditions and can develop into epizootic infectious polyserositis in domestic ducks, mainly young, with a mortality of up to 90% (Sandhu, 2008; Wang et al, 2014; Majhi et al, 2020)
This study aimed at investigating incidence of R. anatipestifer infection in diseased ducks, and determining the antibacterial susceptibility pattern and pathogenicity of the prevalent R. anatipestifer isolates in Assiut Province
The most common encountered signs observed in the examined birds were sinusitis, decrease body weight, locomotor disturbances, nervous signs and arthritis

Summary

Introduction

Among the global leading problems confronting duck industry is Riemerella anatipestifer infection that implicates in acute and chronic conditions and can develop into epizootic infectious polyserositis in domestic ducks, mainly young, with a mortality of up to 90% (Sandhu, 2008; Wang et al, 2014; Majhi et al, 2020). The infected birds show lethargy, nasal discharge, swollen sinuses, dyspnea, diarrhea and neurologic disturbances (Sandhu, 2003; Wu et al, 2020). Only few attempts in Assiut were done by Shahata and Sokkar, (1977); Ibrahim and Shahata, (1991); and Ibrahim and Abd Al-Azeem, (2005) to study this problem in ducks. This study aimed at investigating incidence of R. anatipestifer infection in diseased ducks, and determining the antibacterial susceptibility pattern and pathogenicity of the prevalent R. anatipestifer isolates in Assiut Province

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