Epidemiological and genetic characteristics of swine pseudorabies virus in mainland China between 2012 and 2017

Ying Sun,Hechao Zhu,Yongxiang Tian,Huanchun Chen,Tingting Zhao,Qingyun Liu,Lin Hua,Xibiao Tang,Charles W Stratton,Danna Zhou,Zhong Peng,Bin Wu,Wan Liang

doi:10.7717/peerj.5785

Abstract

The outbreak of pseudorabies (PR) in many Bartha-K61 vaccinated farms in China in late 2011 has seriously damaged the pig industry of one of the largest producers of pork products in the world. To understand the epidemiological characteristics of the pseudorabies virus (PRV) strains currently prevalent in China, a total of 16,256 samples collected from pig farms suspected of PRV infection in 27 Provinces of China between 2012 and 2017 were evaluated for detection of PRV. Since the extensive use of gE-deleted PRV vaccine in China, the PRV-gE was applied for determining wild-type virus infection by PCR. Of the 16,256 samples detected, approximately 1,345 samples were positive for the detection of PRV-gE, yielding an average positive rate of 8.27%. The positive rates of PRV detection from 2012 to 2017 were 11.92% (153/1284), 12.19% (225/1846), 6.70% (169/2523), 11.10% (269/2424), 5.57% (147/2640), and 6.90% (382/5539), respectively. To understand the genetic characteristics of the PRV strains currently circulating, 25 PRV strains isolated from those PRV-gE positive samples were selected for further investigation. Phylogenetic analysis based on gB, gC, and gE showed that PRV strains prevalent in China had a remarkably distinct evolutionary relationship with PRVs from other countries, which might explain the observation that Bartha-K61 vaccine was unable to provide full protection against emergent strains. Sequence alignments identified many amino acid changes within the gB, gC, and gE proteins of the PRVs circulating in China after the outbreak compared to those from other countries or those prevalent in China before the outbreak; those changes also might affect the protective efficacy of previously used vaccines in China, as well as being associated in part with the increased virulence of the current PRV epidemic strains in China.

Highlights

Pseudorabies virus (PRV) mainly causes reproductive failure in sows as well as respiratory and neurological symptoms in piglets (Mettenleiter, 2000; Nauwynck et al, 2007)
Of the 16,256 samples, 1,345 samples were positive for the detection of pseudorabies virus (PRV)-gE, yielding an average positive rate of 8.27%
Higher positivity rates of PRV were detected in January, February, March, April, June, October, November and December; and winter (December, January and February), spring (March, April, and May) and autumn (September, October and November) were the seasons with the high positivity rate of PRV detection during 2012 and 2017 (Figs. 2A and 2B)

Summary

Introduction

Pseudorabies virus (PRV) mainly causes reproductive failure in sows as well as respiratory and neurological symptoms in piglets (Mettenleiter, 2000; Nauwynck et al, 2007). PRV possesses a double-stranded liner DNA genome which contains more than 70 functional genes encoding proteins participating in the formation of viral capsid, tegument, and envelope (Pomeranz, Reynolds & Hengartner, 2005) Among these proteins, the envelope component proteins gB and gC induce cellular and humoral imMune responses (Ober et al, 1998; Ober et al, 2000), while gE acts as a major virulence determinant of PRV to pigs (Kimman et al, 1992; Wang et al, 2014). A number of studies have noted that the Bartha-K61 vaccine appears to be unable to provide full protection against PRV strains isolated from those outbreaks (An et al, 2013; Wang et al, 2014; Yu et al, 2014) These findings suggest that there may be important changes in the PRVs currently circulating in China.

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