Epidemiological and Drug-Resistance Types of Methicillin-Resistant Staphylococcus Aureus Strains Isolated From Surgical and Transplantation Ward Patients During 2010 to 2011

Abstract

BackgroundThe increasing prevalence of multi-drug–resistant methicillin-resistant Staphylococcus aureus (MRSA) is a substantial problem in hospitals worldwide, especially in wards with immunocompromised patients undergoing organ transplant. Epidemiological characteristics and antibiotic susceptibility profiles of hospital-acquired (HA) MRSA strains isolated from surgical/transplantation ward patients were studied. MethodsWe analyzed 26 HA-MRSA strains isolated from 22 patients hospitalized at 3 different surgical and transplantation wards at a Warsaw clinical hospital during 2010 to 2011. Eleven patients were MRSA-asymptomatic carriers. Strain relatedness was evaluated through the use of multi-locus sequence typing (MLST), multi-locus variable-number tandem repeat analysis (MLVA), and random amplified polymorphic DNA/arbitrarily primed PCR (RAPD) methods. Antibiotic susceptibility was assessed the use of routine diagnostic methods. ResultsThe evaluated strains belonged to 4 clonal complexes (CCs) and 4 sequence types (STs): CC30/ST36 (65.4%), CC8/ST8 (15.4%), CC5/ST1827 (11.5%), and CC1/ST1 (7.7%). Six MLVA types and 6 RAPD types were isolated. A ciprofloxacin-, erythromycin-, and clindamycin-resistant CC30/ST36 clone (MLVA type 1, RAPD type 1A) was isolated in all wards. The isolated HA-MRSA strains were most often resistant to ciprofloxacin (100%), erythromycin (96.2%), clindamycin (84.6%), and gentamycin (30.8%). ConclusionsA ciprofloxacin-, erythromycin-, and clindamycin-resistant HA-MRSA ST36 CC30 clone, which prevailed on transplantation wards in the years 2010 to 2011, is probably one of the international epidemic clones named UK EMRSA-16 or USA200.