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Abstract
A study was conducted to determine the prevalence of microfilariasis and analysis of diagnostic methods in buffaloes at Veterinary Poly Clinic, Gudiwada with relation to season, age and breed for a period of 1year (March 2011-February 2012).Out of 1,222 blood smears examination, only 123 samples were found positive for microfilariasis with a prevalence rate of 10.06%. Highest prevalence was observed in monsoon (12.54%) followed by summer (9.18%) and least in winter (7.05%). Buffaloes above 9years age were at high risk with a prevalence rate of 12.5%, followed by 3-9years (10.7%) and below 3years age (6.09%) respectively. Breed wise prevalence of microfilariasis was higher in graded murrah buffaloes 10.87% compared to non-descripts (6.19%). Three commonly used diagnostic techniques viz. Modified Knott's Technique, thick blood smear examination and wet film examination were done to evaluate comparative sensitivity/efficacy. Among these techniques, 100% efficacy was observed in Modified Knott's Technique and 86.99% in thick blood smear examination followed wet film examination 78.04% respectively.
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