Abstract
The fungi present on a sample of retted flax stems were isolated and identified and Epicoccum nigrum was the most frequent colonist. Sterilized, desiccated flax stems retted following inoculation with E. nigrum and stems treated with enzymes extracted from E. nigrum colonized stem tissues also became partially retted. When grown on flax stem tissue E. nigrum produced polysaccharide-degrading enzymes including polygalacturonase, pectin-lyase, cellulase, arabanase and xylanase. The pectic enzymes were the first to reach maximal activities followed by both the cellulase and hemicellulases after a two day lag phase. Release of reducing groups occurred from flax stems treated with extracts of E. nigrum colonized tissue, greatest tissue destruction occurring when cellulase and hemicellulase activities were maximal.
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