Abstract
Introduction Changes in DNA-methylation patterns in alcohol-dependent patients have been described in various studies. However, epigenetic regulation of genes responsible for the ethanol metabolism has not been in the focus of recent research. Objective Aim of our study was to evaluate changes in DNA-methylation of the aldehyde dehydrogenase 2 (ALDH2) in respect to genetic variants (rs886205 ALDH2-SNP) in blood samples of alcohol-dependent patients compared with healthy controls. Methods During an inpatients detoxification treatment we draw blood samples from 82 alcohol-dependent, male patients on day 1, day 7 and day 14. Bisulfite-treated DNA was subjected to methylation analysis via sequencing. Findings of the alcohol-dependent group were compared with results of a healthy control group (N=34) Results Our results show decreasing methylation levels of a repressive ALDH2 promoter fragment to control levels only in homozygous A-allele patients of the rs886205 SNP. Conclusion Findings of this study reveal a specific genetic-epigenetic interaction in ALDH2 regulation which may have crucial importance for the understanding of epigenetic regulation of ethanol metabolism in humans
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