EP08.02-032 High Efficiency Multiplex Detection of Molecular Alterations in Supernatants of Pleural Effusion and Cerebrospinal Fluid

Abstract

A significant percentage of advanced lung cancer (LC) patients show central nervous system (CNS) metastasis or pleural involvement at diagnosis and/or after progression to therapies. In these cases, tumor cells can be often found in cerebrospinal fluid (CSF) or malignant pleural effusion (MPE). Although the detection of tumor derived mutations and gene fusions in the supernatant of MPE or CSF has been documented, the implementation in the routine practice of this type of biological material as a source of circulating free DNA (cfDNA) or RNA (cfRNA) is not standardized.