Abstract
Inoculation of concanavalin-A (Con-A) into the allantoic cavity of chicken embryos resulted in a massive infiltration of eosinophilic granulocytes into the spleens. Immunohistochemical staining of formalin-fixed, paraffin-embedded spleen sections with monoclonal anti-major basic protein (MBP) serum revealed that these cells contain MBP, and were therefore eosinophils. Transmission electron microscopic examination of the spleens showed that these cells contained bi-lobulated nuclei and many granules of various sizes and shapes. These granules, however, did not contain a central core, which is a prominent feature in their mammalian counterparts. The eosinophils isolated from adult chickens underwent remarkable surface changes when incubated with the chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP) by forming and extending surface projections. Adult eosinophils were immotile, but showed chemotactic response when fMLP was used as a chemoattractant in chemotaxis assays.
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