Abstract
The overall aims of the proposal were to identify intermediates in the process of methane formation from acetate. The specific aims were: (1) to characterize the mechanism of acetyl-CoA conversion to CH{sub 3}-H{sub 4}MPT, CO, and CoA and elucidate the mechanism of CO oxidation by the methanosarcina thermophila CO dehydrogenase/acetyl-CoA synthase (CODH/ACS), and (2) to study the mechanism of methyl-CoM reduction to methane by developing an activation protocol for methyl-CoM reductase (MCR) and identifying intermediates in the MCR mechanism. The authors also made significant progress toward accomplishing two other specific aims that were not included in the original proposal. These were: (1) to characterize the M. thermophila heterodisulfide reductase (HDR), and (2) to characterize the methyltetrahydromethanopterin: Coenzyme M methyltransferase from Methanosarcina mazei strain Goe1 in collaboration with Gerhard Gottschalk`s group. Results from these four studies are summarized.
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