Abstract
Abstract A procedure for the concentration of the enzyme phytochelatin synthase from a crude homogenate of Silene vulgaris suspension cells is described. This enzyme catalyzes the formation of the heavy metal complexing phytochelatins from glutathione. Optimization of the incubation parameters (glutathione and Cd 2+ concentration, pH, temperature, time, antimicrobial agents) resulted in conditions that yielded 35 g of phytochelatin peptides ( γ -glu-cys) n -gly (n=2–6) per liter incubation mixture during a 55 day period. The time-space yield was 633 mg PC n ×l −1 ×day −1 . The isolation of total and individual phytochelatin peptides by semipreparative HPLC is described.
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