Abstract
The following enzymic systems capable of reducing Mb + have been studied: 1. 1. DPN reductase isolated from the heart of pig, which reduces Mb + only in the presence of methylene blue. 2. 2. TPN reductase prepared from yeast. 3. 3. Cytochrome b 2 which slowly reduces Mb + both in anaerobic and aerobic conditions. The addition of quinone augments enormously the velocity of the reduction of Mb + by cytochrome b 2. 4. 4. Even the DPNH-quinone and TPNH-quinone systems are able to reduce Mb + in various experimental conditions. A comparative study of reduction velocities by various enzymic systems of cytochrome c, Mb +, and Hb + demonstrates that these three pigments are reduced at different rates. The difference in the velocity of the reduction of Mb + and Hb + represents another characteristic distinction between these two pigments.
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