Enzymic production of feruloyl xylo-oligosaccharides from corn cobs by a family 10 xylanase from Thermoascus aurantiacus

Abstract

Feruloyl xylo-oligosaccharides (FeXOSs) were obtained from corn cobs (CCs) by treatment with a Thermoascus aurantiacus family 10- β- d-endoxylanase. CCs subjected to a sonication (ST-CC) or an autoclave treatment in order to enhance enzymic access and FeXOS production. Enzymic FeXOS production was increased by CCs thermal treatment up to 3.5 times. The kinetic parameters K E and V max, describing enzyme-dependent production rates of FeXOSs from CCs, were estimated at 130±20 nM and 290±10 nM h −1, respectively. The reaction parameters affecting the FeXOS production, such as substrate concentration, enzyme loading and reaction time, have been investigated. The maximum FeXOS production was observed after 24 h incubation of 100 g L −1 AT-CC with 570 nM xylanase. The obtained yield was 12 μmol of FeXOSs per gram of CC.