Abstract
An enzymic procedure is described which utilizes pancreatic cholesterol esterase for the synthesis of unsaturated fatty acid esters of cholesterol in amounts from 1 to 75 mg. The procedure is particularly suitable for the synthesis of very small amounts of labeled, high specific activity cholesteryl esters. A number of cholesteryl-4-C 14 esters were obtained in good yield (from 70 to 75% starting with 1 mg of cholesterol and 50 to 60% starting with 75 mg of cholesterol) and with a degree of purity ranging from 93 to 99%.
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