Abstract
1. 1. Extracts of rabbit-liver acetone powder have seen shown to catalyze the formation of catechol from 3,5-cyclohexadiene-1,2-diol. TPN is required for this dehydrogenation reaction. 2. 2. The mammalian enyme oxidized DIOL and naphtalene,1,2-dihydro-1,2-diol, but had no effect on d-quinic acid, myo-inositol, 2,3-butanediol, glycerol, l-gulonic and galactonic acids. 3. 3. The optimum pH of the reaction was found to be 9.0. Michaelis constants were 2.4·10 −4 M for TPN and 7.05·10 −3 M for DIOL. 4. 4. All attempts to reverse the reaction proved unseccessful. 5. 5.Diol-dehydrogenase activity was also observed in crude extracts of Aerobacter aerogenes. However, the bacterial enzyme required DPN for maximal activity and did not oxidize naphthalene-dihydro-diol.
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