Enzymes of ammonia assimilation in legume nodules: A comparison between ureide‐ and amide‐transporting plants

Abstract

Levels of amide and ureide biogenic enzymes were compared in the plant cytosol fractions of root nodules from soybean (Glycine max L. Merr., cv. Williams), pintobean (Phaseolus vulgaris L. cv. Pinto) and Lupin (Lupinus angustifolius L. cv. Frost). Enzymes of purine oxidation were found to be present in significant quantities only in ureide‐transporting pintobean and soybean nodules. The levels of these enzymes were low in lupin, but this amide‐exporter had significantly higher levels of asparagine synthetase. Enzymes of de novo purine biosynthesis and glycine biosynthesis were present at higher levels in pintobean and soybean, consistent with a role for de novo purine biosynthesis in ureide biogenesis. The low levels of these enzymes in lupin are consistent with a role in general purine and amino acid metabolism in these nodules, not directly related to the synthesis of transport compounds for fixed atmospheric nitrogen. Amino acid concentrations in soybean, pintobean and lupin nodules reflected the metabolic differences between amide and ureide plants. The comparative data presented are consistent with a pathway of ureide biogenesis using glutamine, glutamate and aspartate synthesized via reactions catalyzed by glutamine synthetase, glutamate synthase and aspartate aminotransferase in the de novo synthesis of purines followed by oxidation of these purines to produce the ureides allantoin and allantoic acid.