Abstract

Introduction Extremely Thermostable Enzyme Discovery Direct Purification From Extreme Thermophile Biomass Expression Cloning Sequence Analysis of Genomic DNA Enzymes from Extreme Thermophiles General Characteristics Enzymes from Extreme Thermophiles Involved in Intermediate Metabolism Proteases from Extreme Thermophiles Glycosyl Hydrolases from Extreme Thermophiles Mechanisms of Thermostability Intrinsic Factors Influencing Protein Stability Extrinsic Factors Influencing Protein Stability Recombinant Extremely Thermophilic Enzymes Uses of Extremely Thermostable Enzymes General Considerations Polymerase Chain Reaction Replacements of Existing Industrial Enzymes New Opportunities Summary Bibliography Keywords: archaea; biomass; enzymes; hyperthermophilic; proteases; protein stability; thermostable

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