Enzymes and non-enzymatic antioxidants responses to sequential cold stress in polymorphic noble scallop Chlamys nobilis with different total carotenoids content

Abstract

Noble scallop, an economically important edible marine bivalve displays polymorphism in shells (golden and brown) and flesh colors (orange and white). Mass mortality of noble scallops usually occurs during the winter months. Interestingly, carotenoid-rich golden scallops demonstrated much higher survival rates than brown scallops in winter. In order to understand the response of polymorphic noble scallops to sequential cold stress, the present study aimed to investigate the enzyme and non-enzymatic antioxidant responses of golden and brown scallops under sequential cold stress. Parameters evaluated included total carotenoid content (TCC), fatty acid composition, total antioxidant capacity (TAC), methylenedioxyamphetamine (MDA) content, catalase (CAT) activity, and superoxide dismutase (SOD) enzyme activity. The results of the present study revealed that golden scallops have higher cold tolerance than brown scallops. Golden and brown scallops are well adapted to low water temperature of above 12 °C, but in areas where winter water temperatures are below 12 °C, golden scallops are more suitable for aquaculture than brown scallops. The findings of this study are crucial to understanding the physiological responses of polymorphic scallops to cold stress and identify suitable candidates for winter aquaculture.