Enzyme-linked immunosorbent-assay for Deoxynivalenol (DON).

Fang Ji,Jianhong Xu,Jianrong Shi,Hua Li

doi:10.3390/toxins3080968

Abstract

Deoxynivalenol (DON), one of the trichothecene mycotoxins, is a worldwide contaminant of wheat and barley, especially when infected by Fusarium graminearum, the causative agent of an epidemic wheat disease called Fusarium Head Blight. Because of the high risk of DON ingestion and the possibility of frequent exposure, it is important to develop a rapid and highly sensitive method for easy identification and quantification of DON in grain samples. In this study, we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) to detect DON in wheat. We conjugated 3-O-Hemisuccinyl-DON (3HS-DON) to Bovine serum albumin (BSA) and Ovalbumin (OVA), and obtained DON-specific mice antisera. The indirect competitive ELISA revealed that the optimal concentration of mice serum and the coated antigen was 1/1600 and 1/1500, respectively. The antiserum cross-reacted with the trichothecenes 3-acetyl-DON and T-2 toxin, reaching about 55.2% and 6.3%, respectively, as compared with DON. Results showed that the assay could be performed satisfactorily using an extraction buffer containing less than 15% methanol. Recovery from DON was 82–93% in grains. The linear detection range of DON in grains was between 0.01 and 100 μg/mL.

Highlights

Mycotoxins are toxic metabolites produced by fungi and are of considerable concern as they cause plant diseases but are damaging to human and animal organs when contaminated food is ingested [1,2,3]
Deoxynivalenol (DON) is a common mycotoxin produced by some Fusarium species such as Fusarium graminearum and F. culmorum and is often found in small grains that have been infected with these Fusarium species
The most common small grain disease is called Fusarium head blight (FHB), which often develops following moist environmental conditions when the head is in flower

Summary

Introduction

Mycotoxins are toxic metabolites produced by fungi and are of considerable concern as they cause plant diseases but are damaging to human and animal organs when contaminated food is ingested [1,2,3]. Deoxynivalenol (DON) is a common mycotoxin produced by some Fusarium species such as Fusarium graminearum and F. culmorum and is often found in small grains that have been infected with these Fusarium species. The most common small grain disease is called Fusarium head blight (FHB), which often develops following moist environmental conditions when the head is in flower. The importance of DON to food safety is due to the severe effect that this mycotoxin has on animal systems. DON can interfere with both protein and DNA synthesis in the cell.

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Results

Discussion

Conclusion