Abstract
The enzyme-linked immunosorbent assay (ELISA) was applied to the detection of antibodies to Erysipelothrix rhusiopathiae, and ELISA values were compared with growth agglutination (GA) titers. Ultrasonicated antigen of E. rhusiopathiae strain ‘Agata’ s(serovar: 5) was used as ELISA antigen. GA test was done by using live cells of E. rhusiopathiae strain ‘Marienfelde’ (serovar: la).As compared ELISA values with GA titers of 121 swine sera, the ELISA values had a high correlation with GA titers. The sera of piglets of the same litter were tested by ELISA at 30 to 90 days of age. Maternal antibody titers of piglets of the same litter varied greatly and antibodies were not detected in the sera of piglets at more than 50 days of age. The present investigation demonstrated that the ELISA was effective for the detection of antibody to E. rhusiopathiae, and ELISA values were correlated with GA titers. These results indicate that the vaccination of attenuated strain of E. rhusiopathiaeto piglets should be carried out in consideration of the period that maternal antibodies are lost.
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