Abstract
Kinetics of ion exchange separation of serine alkaline protease (SAP) by cation- and anion-exchangers, respectively, for the SAP +/H + and SAP −/OH − ion exchange reactions were studied with four different resins, i.e. low acidic Macro-prep CM, high acidic Macro-prep HighS, low basic Macro-prep DEAE, and high basic Macro-prep HighQ. The effects of pH on ion exchange equilibria were investigated within pH=6.5–8.0 and pH=9.5–11.0, respectively, for the SAP +/H + cation exchange and SAP −/OH − anion exchange reactions at T=10–25 °C, and the results were interpreted on the basis of the theory of exchange of enzyme-ions. Although Macro-prep CM showed the highest SAP binding capacity at pH=7.0, maximum recovery of SAP activity was obtained at 15 °C and pH=6.5. Effects of feed concentration and flow rate were investigated in differential-(DIC) and integral (IC) ion exchange columns and 0.025 mol dm −3 and 1.0×10 −2 dm 3 min −1 gave the highest enzyme recovery as 70%. The overall internal–external mass transfer coefficient which was found by differential analysis showed the importance of liquid phase mass transfer resistances on the process under the operation conditions applied.
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