Enzyme-based green synthesis, characterisation, and toxicity studies of cellulose nanocrystals/fibres produced from the Vetiveria zizanioides roots agro-waste.

Abstract

Vetiver zizanioides roots are considered the most useful part of the plant. It is widely used to extract oil. The aromatic oil is used in perfumery, food-flavouringand cosmetic industries. However, presently, there are no reports available for the usage of vetiver roots agro-waste after oil extraction in nano-based products. Considering the concept of value-added products and green-chemistry approaches, synthesising cellulose nanoparticles (CNPs) using enzymatic treatment from agro-waste has emerged as a viable option. CNP's non-toxicity, biodegradability, and biocompatibility have sparked the industry's interest in its production. Therefore, in the present study, 3 enzymes, cellulase, pectinase, and viscozymes, were used for the green synthesis of CNP. The characterisation of CNP was done using techniques like DLS, FTIR, TEM, SEM, AFM, and TG/DTG, and cytotoxicity of CNP was studied in human skin cell-line (HaCaT) using MTT assay. Results show that CNPs synthesised using viscozyme and pectinase were of crystalline nature (2.0-3.0nm) and cellulase were of fibres (40-60nm). The FTIR confirmed that CNPs were devoid of lignin/hemicellulose. The AFM pictures revealed thick and thin nanoparticles with a variety of morphologies. The thermal stability of cellulose was higher compared to CNP. All the synthesised CNPs were crystaline, with a 60-70% crystallinity index. Furthermore, CNP did not show cytotoxic effect on HaCaT cells upto 500µg/mL concentrations. In conclusion, pectinase and viscosyme may be used for synthesing cellulose-nanocrystals and cellulase enzyme for cellulose-nanofibers from the vetiver roots agro-waste. The findings revealed that Vetiveria zizanioides agro-waste-derived CNP is a sustainable material that can be used as a reinforcing agent/nanocarrier in textile and drug-delivery systems.