Abstract

Liver and striated muscle glycogen particles, both isolated and in tissue sections, were examined in the electron microscope. They were treated enzymatically with diastase, amylase, and pepsin. Pepsin was used because it is believed that glycogen in tissues is bound to proteins. The enzymatic treatment of ultrathin sections gave the following results: (a) Liver glycogen particles disappeared from the sections after digestion with any one of the enzymes used; (b) muscle glycogen particles did not disappear from the sections even after a double treatment with pepsin and diastase. Glycogen particles isolated from either liver or striated muscle were digested by diastase. Pieces of liver and muscle, incubated in 0.1% glucose to synthesize more glycogen, contained glycogen particles which appeared larger than usual, as seen in the electron microscope. Following diastase treatment, these particles returned to their original dimensions in muscle sections, and disappeared completely from the liver sections.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.