Abstract
A histochemical technique that has been employed on guinea pig cochlea for demonstration of the enzyme, succinic acid dehydrogenase, will be described. The method employs the conventional chemical agent Nitro BT to develop a blue diformazan which is deposited at the enzyme site in the form of fine rods and granules. Since this enzyme is involved only in the Krebs cycle, its presence in a cell indicates oxidative metabolism taking place at that point. The stain developed is proportional to the quantity of enzyme present, so it is possible to draw conclusions as to the relative metabolic rates of various cells within an organ. Because the cochlea is completely surrounded by bone, a technique of direct perfusion of the cochlea via scala vestibuli and scala tympani was developed to bring the substrate into closer contact with cochlear structures. Slides demonstrating the distribution of this enzyme in the guinea pig cochlea will be shown.
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