Abstract
The role of unsaturated fatty-acid starvation, and of the substitution of trans for cis fatty acids in the membrane phospholipid, on the secretion of alkaline phosphatase, has been investigated. A system in which alkaline phosphatase synthesis was initiated by a temperature shift has been used to obviate possible complications resulting from phosphate depletion. In contrast to earlier reports, we find (a) there is very little effect of unsaturated fatty-acid starvation on the synthesis of alkaline phosphatase; (b) the synthesis of both ฮฒ-galactosidase and alkaline phosphatase synthesis was severely reduced below 27โ30ยฐC in cells grown on trans ฮ 9 16:1 fatty-acid, compared to cells grown on the cis ฮ 9 16:1 analogue. Thus no preferential effect on alkaline phosphatase synthesis was observed.
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