Enzyme-regulated NO programmed to release from hydrogel-forming microneedles with endogenous/photodynamic synergistic antibacterial for diabetic wound healing

Abstract

The infection-prone wound pathology microenvironment leads to ulceration and difficult healing of diabetic wounds, which seriously affects the quality of survival of patients. In this study, natural polymer materials gelatin and polylysine were used as substrates. By introducing iron/tannic acid (FeIIITA) composite nanoparticles with excellent photothermal properties into the system, the glutamine residues of gelatin were crosslinked with the primary ammonia of polylysine by glutamine aminotransferase. A nanocomposite hydrogel with excellent antibacterial ability and NO production was constructed it was used to improve the clinical problems of diabetes wounds that were difficult to vascularize and easy to be infected. Under the premise of maintaining its structural stability, the hydrogel can be customized to meet the needs of different mechanical strengths by adjusting the ratios to match different diabetic wounds. Meanwhile, the photothermal effect of FeIIITA nanoparticles can synergize with the endogenous antibacterial ability of polylysine to improve the antibacterial efficacy of hydrogels. The potential of hydrogel to promote intracellular NO production was confirmed by fluorescent staining. Microneedle patches prepared from hydrogel can be applied to diabetic wounds, which can achieve NO deep release. Its anti-inflammatory and angiogenic abilities are also useful in achieving effective healing of diabetic wounds.