Enzyme-linked immunosorbent assay of mumps antibody for evaluation of immune status before and after vaccination.

Abstract

Legend. ODs of ELISA of mumps IgG antibodies in sera of recipients of measles-rubella-mumps combined live vaccine. ODs of postvaccination sera are shown as a function of those of prevaccination sera. Vaccines, consisting of (1) measles: AIK-C 557 (Kitasato Laboratory); (2) rubella: TO 336 (Takeda Chemical Industries); and (3) mumps: Torii strain (Takeda Chemical Industries), were injected into 104 children from one to five years of age who had no history of mumps infection. Serum samples were taken at the time of vaccination and six weeks after vaccination. A microtitration plate (IMMULON(R); Dynatech Laboratories, Alexandria, Va) was pretreated with 10070 fetal calf serum in PBS (pH 7.3) for 1 hr and then washed three times in PBS. Purified mumps antigen (supplied by the Takeda Chemical Industries) diluted with 0.05 M carbonate buffer (pH 9.6) was applied. The plate was incubated overnight at 4 C and washed three times. Subsequent steps of tile further procedure were the same as those described previously [1]. Variations between plates were corrected using internal standards (i.e., three different dilutions of mumps hyperimmune IgG). Since the coefficient of variation of this method was 10%, increases of OD >30% were considered to be significant. Summary