Abstract
Tumor necrosis factor (TNF), a monokine released mainly by macrophages, has the ability to kill cells, especially tumor cells [l]. Since the first report by Carswell et al in 1975 [2], many researchers have given a great deal of attention to this factor because of its potential for use in cancer therapy. Assays for TNF have mainly been by bioassay using L929 cells [3,4], but this approach is not suited to human sera because of assay imprecision. In order to overcome this problem, we developed an ELISA for hTNF.
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