Abstract
A novel graphical method for analysing the rates of enzyme reactions to obtain kinetic parameters K i, K i′ and β (degree of partiality) and to identify the types of inhibition and the enzyme mechanisms is described. The method consists of plotting experimental data as v (V o −v) versus 1 (I) at different substrate concentrations. I is the inhibitor concentration, V o and v are the rates of enzyme reaction attained by the system in the presence of a fixed amount of substrate and in the absence and presence of inhibitor respectively. Complete inhibition gives a single straight line that passes through the origin while partial inhibition gives a straight line that intercepts the 1 (I) axis at a point away from the origin.
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