Abstract
Soybean seedlings ( Glycine max, cv. Amsoy 71) were inoculated in the hypocotyls with mycelium from either race 1 (incompatible) or race 3 (compatible) of Phytophthora megasperma f. sp. glycinea, and the activities of phenylalanine ammonia-lyase (PAL), chalcone synthase, glucose-6-phosphate dehydrogenase, and glutamate dehydrogenase determined at various times after inoculation. A drastic increase in activity with a subsequent decrease occurred with all enzymes, whereas wound controls showed no or very little change in activity. A higher activity of PAL was reached in the incompatible than in the compatible interaction. Rates of PAL synthesis were determined by pulse-labeling with l-[ 35S]methionine and immunoprecipitation of the enzyme. These experiments prove that increase in PAL activity is due to de novo synthesis of the enzyme.
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