Enzyme immunoassays for 15-F2T isoprostane-M, an urinary biomarker for oxidant stress.

Abstract

Isoprostane assays have significant potential for use as molecular biomarkers of human oxidant stress (Morrow et al. 1999, Lawson et al. 1999). Recent studies indicate that: (a) significant amounts of F2 isoprostanes derived from systemic oxidant stress are metabolized before excretion, (b) a significant quantity of free F2 isoprostanes are produced by the kidney, so free isoprostanes detected in urine may not accurately reflect systemic oxidant stress and (c) 2,3-dinor-5,6-dihydro-15-F2tIsoprostane (F2-IsoP-M, IsoP-M), a major urinary metabolite of systemic 8-iso-PG F2Œ, has recently been identified (Figure 1). Urinary F2-IsoP-M may, therefore, prove to be an excellent marker for systemic oxidant stress. Our goal is to develop a sensitive and specific immunoassay for F2-IsoP-M, which should provide a simple non-invasive method for routine quantitative assessment of human oxidant stress.