Abstract
A sensitive enzyme immunoassay for the determination of monensin has been developed. Horseradish poroxidase (HRP) used as a labeling enzyme was conjugated with monensin according to the mixed anhydride method. Bound and free fractions after immune reaction were separateb by double antibody solid phase using Sepharose 4B. Three assay methods, spectrophotometric, fluorophotometric and luminometric, for the measurement of HRP activity were compared. Spectrophotometric method using ABTS [2, 2'-azino-di-(3-ethyl-benzthiazoline-sulfate (6))] was suitable for routine assay method. Enzyme immunoassay developed here appears to be applicable to the determination of monensin in various tissues.
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