Enzyme Histochemistry of Hemal Nodes of Indian Buffalo (Bubalus bubalis)

Abstract

The present study was conducted on hemal nodes of buffalo to study the histoenzymic distribution of phosphatases and oxidoreductases. The fresh unfixed buffalo hemal nodes were immediately collected after sacrifice and were subjected to cryostat sectioning at -200 C with cryostat microtome. The sections were incubated with substrates for demonstration of various enzymes. The study revealed that in both the groups, weak alkaline phosphatase (AKPase) activity was observed in capsule of the hemal node. The AKPase activity was moderate in subcapsular sinus, periphery of lymphoid follicles and diffused lymphocytes. Uniform weak Glucose 6-phosphate activity was observed in entire hemal node. Succinic dehydrogenase (SDH) activity was moderate in capsule and lymphoid follicles and weak in subcapsular sinuses. Lactic dehydrogenase activity was moderate to strong in capsule, sub-capsular sinus and peripheral area of lymphoid follicles. Nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-D) and reduced Nicotinamide adenine dinucleotide diaphorase (NADH-D) was weak to moderate in the capsule and moderate to strong in lymphoid follicles. The presence of variable activity of different enzymes in hemal nodes was correlated with maturation of lymphocytes and development of different metabolic pathways.