Enzyme-histochemical differences in fibre-type between the human major and minor zygomatic and the first dorsal interosseus muscles

Abstract

Human masticatory muscles, innervated by the trigeminal nerve, differ in fibre-type composition from limb and trunk muscles, but the anterior and the posterior belly of the human digastric muscle, innervated by the trigeminal and facial nerves, respectively, do not. The major and minor zygomatic muscles from adult males, which originate from the second branchial arch and are supplied by the facial nerve, were analysed enzyme-histochemically and compared with the first dorsal interosseus hand muscle, which has spinal innervation and, like the masticatory and facial muscles, a large cortical representation. Both zygomatic muscles had a marked predominance of type II fibres, the minor one having the largest proportion (89.1 per cent) ever reported in human skeletal muscle. Besides type I, IIA, IIB, and a few type IIC fibres, there was a large group with an ATPase reaction at pH 4.6, between that of type IIA and type IIB, and termed IIAB. This fibre-type profile may reflect a special isomyosin composition. Type I and II fibres were of about equal diameter, corresponding to that of type I fibres in the masticatory muscles. Individual and intra-muscular variability in fibre size and shape was considerable. The unusually high frequency of type II fibres in the zygomatic muscles suggests that they have fast-contraction properties and relatively large motor units, and therefore are poorly adapted to finely-graded movements. The absence of muscle spindles supports this view. The hand muscle had a chequer-board pattern of type I, IIA and IIB fibres, similar to that of large limb and trunk muscles, with no difference between its two heads. Its predominance of type I fibres, and the occurrence of spindles indicates a capacity for finely-graded activity and resistance to fatique. These differences between the facial, masticatory, digastric and hand muscles imply that functional requirements are of greater importance for muscle differentiation than embryologic origin and nerve supply.