Abstract
Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix. Avoiding such damage would improve cell culture efficiency. Here we propose an enzyme-free cell detachment method that employs the acoustic pressure, sloshing in serum-free medium from intermittent traveling wave. This method detaches 96.2% of the cells, and increases its transfer yield to 130% of conventional methods for 48 h, compared to the number of cells detached by trypsinization. We show the elimination of trypsinization reduces cell damage, improving the survival of the detached cells. Acoustic pressure applied to the cells and media sloshing from the intermittent traveling wave were identified as the most important factors leading to cell detachment. This proposed method will improve biopharmaceutical production by expediting the amplification of tissue-cultured cells through a more efficient transfer process.
Highlights
IntroductionTrypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix
Cell detachment is essential in culturing adherent cells
The cells used in this study were able to proliferate with only the adhesive proteins they already possess: supplementation by serum was unnecessary and the proliferation was similar whether Rid = 0.875 or 1
Summary
Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix Avoiding such damage would improve cell culture efficiency. We propose an enzyme-free cell detachment method that employs the acoustic pressure, sloshing in serum-free medium from intermittent traveling wave. Acoustic pressure applied to the cells and media sloshing from the intermittent traveling wave were identified as the most important factors leading to cell detachment This proposed method will improve biopharmaceutical production by expediting the amplification of tissue-cultured cells through a more efficient transfer process. When the temperature is lowered, these surfaces are rapidly hydrated, become hydrophilic, and cause spontaneous cell release This method has been used to generate cell sheets[21] and tissues[22]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
